Figure 2

Proteome mapping of mouse liver. A pre-fractionation step prior to the IEF was carried out for the samples 1A-6A to separate proteins in two fractions: acid proteins with pH values < 7 (1A-a; 6A-a) and basic proteins having pH values > 7 (1A-b; 6A-b). All fractions were run in duplicate modus for the first and second dimensions. In summary, three experiments (E = 1, E = 3, E = 4) were repeated for the samples in the pH range 5-8. One experiment (E = 2) was carried out using the pH range 7-10. A total of 24 gels (grey small boxes) were used for cutting, digestion and MS identification of the spots/proteins. (N° = number; E = experiment; A-a = extract in LB2, acidic fraction; A-b = extract in LB3, basic fraction).