Table 2 Proteins identified which show a 5-fold up- or down-regulation under temperature stress at 47.5°C in the stress bioreactor compared to the reference system, the growth reactor at 37°C.
From: Proteome analysis of the Escherichia coli heat shock response under steady-state conditions
| Â | Â | Â | Â | normalized spot intensity | |
|---|---|---|---|---|---|
protein group | description | function | accession number | growth bioreactor | stress bioreactor |
enzymes involved in oxidative stress | 37°C | 47.5°C | |||
Dps | DNA-binding protein | protection of DNA | NP_415333.1 | 1 ± 0.024 | 7.32 ± 0.479 |
AhpC | reductase subunit C | formation of H2O from H2O2 | NP_415138.1 | 1 ± 0.068 | 17.74 ± 1.203 |
SodA | superoxide dismutase | convertion of O2- to H2O2 | NP_418344.3 | 1 ± 0.006 | 17.74 ± 0.11 |
proteins of the cell envelope | |||||
FnlC | l-fucosamine synthetase | O antigen biosynthesis | Q5ISY2 | 20.5 ± 4.24 | 1 ± 0.707 |
OmpA | outer membrane protein A | membrane transport | NP_415477.1 | 42 ± 0.004 | 2 ± 0.606 |
OmpF | outer membrane protein F | membrane transport | NP_415449.1 | 1 ± 0.188 | 31.47 ± 1.425 |
OmpF | outer membrane protein C+F | membrane transport | NP_415449.1 | 1 ± 0.078 | 28.47 ± 2.21 |
Ag43 | antigen 43 | autoaggregation | AP_002599.1 | 1 ± 0.324 | 7.57 ± 10.212 |
chaperones and proteins involved in protein biosynthesis | |||||
Skp | chaperone protein | periplasmic chaperone | NP_414720.1 | 1 ± 0.707 | 33.92 ± 0.354 |
OppA | oligopeptide-binding protein | chaperone | NP_415759.1 | 8.77 ± 0.362 | 1 ± 0.099 |
Hsp40 | chaperone protein | cytoplasmic chaperone | NP_414556.1 | 1 ± 0.098 | 5.45 ± 0.536 |
LeuRS | leucyl-tRNA synthetase | linking the amino acid to its tRNA | NP_415175.1 | 1 ± 0.046 | 4.67 ± 0.213 |
RP S2 | ribosomal protein S2 | translation | NP_414711.1 | 16.51 ± 3.29 | 1 ± 0.164 |
RP S4 | ribosomal protein S4 | translation | NP_417755.1 | 1 ± 0.561 | 7.4 ± 1.212 |
Rp L5 | ribosomal protein L5 | translation | NP_417767.1 | 1 ± 0.326 | 5.45 ± 0.427 |
Rp S11 | ribosomal protein S11 | translation | NP_417756.1 | 1 ± 0.09 | 6.53 ± 1.65 |
enzymes involved in the glycolysis, TCA cycle and mixed acid fermentation | |||||
TpiA | triosephosphate isomerase | glycolysis | NP_418354.1 | 1 ± 0.062 | 6.4 ± 0.4 |
GpmA | 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | glycolysis | NP_415276.1 | 1 ± 0.068 | 16 ± 1.09 |
GltA | citrate synthase | TCA cycle | NP_415248.1 | 1 ± 0.036 | 7.88 ± 0.28 |
AcnB | malate synthase | TCA/glyoxylate cycle | NP_414660.1 | 1 ± 0.25 | 0 |
AceB | aconitase B | glyoxylate cycle | NP_418438.1 | 1 ± 0.311 | 5.55 ± 1.73 |
Pta | acetyl-CoA:Pi acetyltransferase | acetate formation and dissimilation | NP_416800.1 | 1 ± 0.25 | 0 |
AtpG | F0F1 ATP synthase subunit gamma | ATP synthesis | NP_418189.1 | 1 ± 0.413 | 7.05 ± 0.48 |
enzymes involved in amino acid biosynthesis | |||||
MetE | triglutamate-homoserine methyltransferase | methionine biosynthesis | NP_418273.1 | 1.04 ± 0.199 | 9.74 ± 0.282 |
FolE | GTP cyclohydrolase I | tetrahydrofolate biosynthesis | NP_416658.1 | 20.33 ± 0.471 | 3.33 ± 0.943 |
CysP | thiosulfate transporter | uptake of sulphate and thiosulfate | NP_4168920.1 | 1 ± 0.188 | 4.17 ± 2.71 |
MetN | DL-methionine transporter subunit N | methionine transporter | NP_414741.1 | 1 ± 0.076 | 15.86 ± 1.21 |
GlyA | serine hydroxy-methyltransferase | serine-glycine biosynthesis | NP_417046.1 | 1 ± 0.149 | 5.56 ± 0.829 |
SerA | phosphoglycerate dehydrogenase | serine-glycine biosynthesis | NP_417388.1 | 1 ± 0.025 | 25.5 ± 0.647 |
PepA | leucyl aminopeptidase | aminopeptidase | NP_418681.1 | 1 ± 0.295 | 5.42 ± 0.943 |