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Table 2 Proteins identified which show a 5-fold up- or down-regulation under temperature stress at 47.5°C in the stress bioreactor compared to the reference system, the growth reactor at 37°C.

From: Proteome analysis of the Escherichia coli heat shock response under steady-state conditions

     normalized spot intensity
protein group description function accession number growth bioreactor stress bioreactor
enzymes involved in oxidative stress 37°C 47.5°C
Dps DNA-binding protein protection of DNA NP_415333.1 1 ± 0.024 7.32 ± 0.479
AhpC reductase subunit C formation of H2O from H2O2 NP_415138.1 1 ± 0.068 17.74 ± 1.203
SodA superoxide dismutase convertion of O2- to H2O2 NP_418344.3 1 ± 0.006 17.74 ± 0.11
proteins of the cell envelope
FnlC l-fucosamine synthetase O antigen biosynthesis Q5ISY2 20.5 ± 4.24 1 ± 0.707
OmpA outer membrane protein A membrane transport NP_415477.1 42 ± 0.004 2 ± 0.606
OmpF outer membrane protein F membrane transport NP_415449.1 1 ± 0.188 31.47 ± 1.425
OmpF outer membrane protein C+F membrane transport NP_415449.1 1 ± 0.078 28.47 ± 2.21
Ag43 antigen 43 autoaggregation AP_002599.1 1 ± 0.324 7.57 ± 10.212
chaperones and proteins involved in protein biosynthesis
Skp chaperone protein periplasmic chaperone NP_414720.1 1 ± 0.707 33.92 ± 0.354
OppA oligopeptide-binding protein chaperone NP_415759.1 8.77 ± 0.362 1 ± 0.099
Hsp40 chaperone protein cytoplasmic chaperone NP_414556.1 1 ± 0.098 5.45 ± 0.536
LeuRS leucyl-tRNA synthetase linking the amino acid to its tRNA NP_415175.1 1 ± 0.046 4.67 ± 0.213
RP S2 ribosomal protein S2 translation NP_414711.1 16.51 ± 3.29 1 ± 0.164
RP S4 ribosomal protein S4 translation NP_417755.1 1 ± 0.561 7.4 ± 1.212
Rp L5 ribosomal protein L5 translation NP_417767.1 1 ± 0.326 5.45 ± 0.427
Rp S11 ribosomal protein S11 translation NP_417756.1 1 ± 0.09 6.53 ± 1.65
enzymes involved in the glycolysis, TCA cycle and mixed acid fermentation
TpiA triosephosphate isomerase glycolysis NP_418354.1 1 ± 0.062 6.4 ± 0.4
GpmA 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase glycolysis NP_415276.1 1 ± 0.068 16 ± 1.09
GltA citrate synthase TCA cycle NP_415248.1 1 ± 0.036 7.88 ± 0.28
AcnB malate synthase TCA/glyoxylate cycle NP_414660.1 1 ± 0.25 0
AceB aconitase B glyoxylate cycle NP_418438.1 1 ± 0.311 5.55 ± 1.73
Pta acetyl-CoA:Pi acetyltransferase acetate formation and dissimilation NP_416800.1 1 ± 0.25 0
AtpG F0F1 ATP synthase subunit gamma ATP synthesis NP_418189.1 1 ± 0.413 7.05 ± 0.48
enzymes involved in amino acid biosynthesis
MetE triglutamate-homoserine methyltransferase methionine biosynthesis NP_418273.1 1.04 ± 0.199 9.74 ± 0.282
FolE GTP cyclohydrolase I tetrahydrofolate biosynthesis NP_416658.1 20.33 ± 0.471 3.33 ± 0.943
CysP thiosulfate transporter uptake of sulphate and thiosulfate NP_4168920.1 1 ± 0.188 4.17 ± 2.71
MetN DL-methionine transporter subunit N methionine transporter NP_414741.1 1 ± 0.076 15.86 ± 1.21
GlyA serine hydroxy-methyltransferase serine-glycine biosynthesis NP_417046.1 1 ± 0.149 5.56 ± 0.829
SerA phosphoglycerate dehydrogenase serine-glycine biosynthesis NP_417388.1 1 ± 0.025 25.5 ± 0.647
PepA leucyl aminopeptidase aminopeptidase NP_418681.1 1 ± 0.295 5.42 ± 0.943