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Figure 2 | Proteome Science

Figure 2

From: Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius

Figure 2

Optimization of extraction buffers for the solubilization of bursa of Fabricius proteins. The total number of protein spots detected in gels is buffer I, 1339 ± 81 spots, II, 1786 ± 103 spots, III, 1702 ± 114 spots, IV, 1851 ± 65 spots, and V, 1792 ± 137 spots. The separation of soluble proteins was performed on 24 cm strips over isoelectric point (pI) gradient of 5-8. Each gel was loaded with 200 μg of total protein and silver stained.

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