Figure 2From: Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius Optimization of extraction buffers for the solubilization of bursa of Fabricius proteins. The total number of protein spots detected in gels is buffer I, 1339 ± 81 spots, II, 1786 ± 103 spots, III, 1702 ± 114 spots, IV, 1851 ± 65 spots, and V, 1792 ± 137 spots. The separation of soluble proteins was performed on 24 cm strips over isoelectric point (pI) gradient of 5-8. Each gel was loaded with 200 μg of total protein and silver stained.Back to article page