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Table 2 Putative identification of protein spots in chicken bursa of Fabricius extracted with method C and extraction buffer IV

From: Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius

Spot No.a

Protein Name

Accession

No.b

M r (kDa)

(pred/exp)c

pI

(pred/exp)

Matched/

unmatchedd

Protein score/best ion scoree

Peptides Identified

S1

Endoplas-mic reticulum protein ERp29

gi| 67476967

25.36/25.18

7.66/5.34

4/61

132/110

ILEQGEEFAANEVVR

S2

lamin B2

gi| 45384202

67.90/21.79

5.31/7.00

22/77

207/60

IKDLEVLFHR

S3

Proteas-ome 26S subunit, non-ATPase, 14

gi| 74004398

27.13/32.3

6.12/6.65

8/60

96/51

LINANMMVLGHEPR

  1. aSpot No. is the unique number which refers to the labels in Fig. 5.
  2. bAccession no. is the MASCOT results of MALDI-TOF/TOF searched from the NCBInr database.
  3. cpred/exp, predicted/experimental.
  4. dThe number of peaks which match/unmatch to the trypsin peptides.
  5. eProtein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification. The proteins had statistically significant protein score of greater than 72 (p ≤ 0.05) were considered successfully identified.