Analysis of plasma fractions. (A) Workflow A, NG fraction separated by CAX. Lanes: 1, NG fraction; 2, CAX flow-through fraction; 3-8, CAX eluates with 100, 200, 300, 400, 500 or 600 mM NaCl. The boxed bands NG-1, NG-2, NG-3 and NG-4 were identified by mass spectrometry. (B) DIGE images of (1) glycosylated and (2) non-glycosylated protein enriched fractions resolved by 2DGE. The proteins in spots P1 to P14 were identified by mass spectrometry and are shown in Table 1.