Validation of representative biomarkers by EIA. The EIAs were carried out as described in Methods. (A) Plasma levels of transthyretin were estimated for various groups of hepatitis patients and controls. The p values shown are based on the Mann-Whitney test; the significance remains the same with the Student's t-test. (B) The levels of alpha-1-microglobulin in urine were estimated for various groups of hepatitis patients and controls. The p-values shown are based on both parametric (Student's t test) and non-parametric (Mann-Whitney U test) tests. All p values shown are relative to Group 5, except in (B) in which Groups 1 and 3 were also compared.