Figure 1

2D-Maps representing total protein extract from macrophages and protein fractions derived from the proposed protocol. 2D mini gels of a total protein extract from macrophages cells (panel on the left) and the 4 fractions obtained by applying the extraction protocol with Triton X114 (cytosolic fraction, membrane fraction, membrane associated fraction and secretome fractions, from the top to the bottom). All the 2D-PAGE are run on a 3–10 non linear IPG strip and an equal amount of total protein content was loaded on each gel. For each 2D map the spots count, as obtained with PDQuest software, is indicated.