High resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics

Polati, Rita; Castagna, Annalisa; Bossi, Alessandra; Campostrini, Natascia; Zaninotto, Federica; Timperio, Anna Maria; Zolla, Lello; Olivieri, Oliviero; Corrocher, Roberto; Girelli, Domenico

doi:10.1186/1477-5956-7-4

Proteome Science

Table 1 membrane fraction identified proteins

From: High resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics

SSP	Mw, kDa theor./exp.	pI predict./exp.	No. of peptides identified	Mascot Score	NCBI Accession Number	Protein ID
5	51372	8.88	11	592	gi\|872121	isocitrate dehydrogenase (NADP+) [Homo sapiens]
	38780	7.57	21	1301	gi\|18645167	Annexin A2 [Homo sapiens]
21	38639	6.32	3	162	gi\|190200	Porin [Homo sapiens]
	42128	5.22	4	198	gi\|28336	mutant beta-actin (beta'-actin) [Homo sapiens]
17	18537	5.21	2	70	gi\|5453559	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d isoform a [Homo sapiens]
	58411	7.58	17	1101	gi\|35505	pyruvate kinase [Homo sapiens]
13	59828	9.16	2	113	gi\|4757810	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit precursor [Homo sapiens]
1	53738	5.03	36	1940	gi\|340219	Vimentin [Homo sapiens]
14	42080	5.37	6	317	gi\|62897625	beta actin variant [Homo sapiens]
15	12905	5.77	3	70	gi\|34616	beta-2 microglobulin [Homo sapiens]
12	59785	9.07	20	1136	gi\|127798841	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle [Homo sapiens]
10	29843	5.57	9	485	gi\|4505773	prohibitin [Homo sapiens]
3	50810	5.02	25	651	gi\|37492	alpha-tubulin [Homo sapiens]
18	30737	8.63	7	420	gi\|238427	Porin 31HM [human, skeletal muscle membranes, Peptide, 282 aa] [Homo sapiens]
11	30337	6.99	3	178	gi\|4758788	NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30 kDa (NADH-coenzyme Q reductase) [Homo sapiens]
20	49851	7.70	20	710	gi\|704416	elongation factor Tu [Homo sapiens]
16	42052	5.29	5	131	gi\|4501885	beta actin [Homo sapiens]
7	42080	5.37	8	398	gi\|62897625	beta actin variant [Homo sapiens]
2	48083	4.95	17	1052	gi\|89574029	mitochondrial ATP synthase, H+ transporting F1 complex beta subunit [Homo sapiens]
8	53559	5.93	4	189	gi\|32709	IFP53 [Homo sapiens]
6	42080	5.37	8	371	gi\|62897625	beta actin variant [Homo sapiens]
4	53297	5.94	6	241	gi\|46593007	ubiquinol-cytochrome c reductase core protein I [Homo sapiens]
19	53809	6.03	19	1278	gi\|40889610	Chain A, Crystal Structure Of Human Tryptophanyl-Trna Synthetase [Homo sapiens]

Identified proteins from membrane fraction. The corresponding standard spot number (SSP) and the parameters of identifications are also indicated.

Back to article page

ISSN: 1477-5956

Contact us

General enquiries: journalsubmissions@springernature.com