Representative 2-DE gel images showing the proteomes of (A) pre-competent larvae, (B) competent larvae, and (C) newly-metamorphosed juveniles of Pseudopolydora vexillosa. The 1st dimension was performed on pH 3-10 linear IPG strips; the second dimension was run on 12.5% acrylamide gels. Gels were stained with Colloidal Coomassie Blue stain. The protein load per gel was about 400 μg. Marked protein spots were identified by MS and their details are listed in Table 1.