Protein spot display for a high M
fraction derived from alkaline membrane extraction of Y. pestis KIM6+ cells grown to stationary phase at 26°C. Proteins isolated from the hpH-MBR fraction were loaded onto a Superdex 200 column (1.6 × 100 cm), separated in the presence of 0.05% Triton X-100, concentrated to ca. 1 mg/mL and lyophilized for 2D gel analysis. The fraction displayed here corresponded to a native protein Mr range from ca. 250 to 450 kDa. Spot numbers are equivalent to those denoted in Additional File 2.