Table 3 List of the differentially expressed protein spots in 2DE of HIV-positive and healthy donors identified by ESI-MS or both ESI-MS and MALDI-TOF/TOF.
From: Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins
spota | Accession NO.b | Protein description | MWc | pI | Scored | Cov.e | Abundance HIV/NOR | Functionf | Locationg |
|---|---|---|---|---|---|---|---|---|---|
1 | KPYM_HUMAN | Pyruvate kinase isozymes M1/M2 - Homo sapiens (Human) | 58470 | 7.96 | 173 | 15% | ↑ | enzyme | Cytosol |
2 | TLN1_HUMAN | Talin-1 - Homo sapiens (Human) | 271766 | 5.77 | 44 | 2% | ↑ | cell-cell junction | Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane. |
3 | LDHB_HUMAN | L-lactate dehydrogenase B chain - Homo sapiens (Human) | 36900 | 5.71 | 316 | 30% | ↑ | enzyme | Cytoplasm. |
4, 14, 15 | CAP1_HUMAN | Adenylyl cyclase-associated protein 1 - Homo sapiens (Human) | 52222 | 8.27 | 129 | 16% | ↑ | actin binding | Cell membrane; PM |
5, 13, 16, 19 | ENOA_HUMAN | Alpha-enolase - Homo sapiens (Human) | 47481 | 7.01 | 217 | 34% | ↑ | Enzyme | Cell membrane; Cytoplasm; Membrane; Nucleus. |
6 | EHD3_HUMAN | EH domain-containing protein 3 - Homo sapiens (Human) | 61971 | 6.06 | 285 | 36% | ↑ | Binding/transport | Cell membrane; Endosome. Membrane |
7 | COR1C_HUMAN | Coronin-1C - Homo sapiens (Human) | 53899 | 6.65 | 253 | 18% | ↑ | Actin-binding/transport | actin cytoskeleton |
8 | ST1A3_HUMAN | Sulfotransferase 1A3/1A4 - Homo sapiens (Human) | 34288 | 5.68 | 85 | 23% | ↑ | Actin-binding/transport | Cytoplasm |
9 | FLNA_HUMAN | Filamin-A - Homo sapiens (Human) | 283301 | 5.7 | 172 | 9% | ↑ | Actin-binding | Cytoplasm; Cytoskeleton; plasma membrane. |
10 | VINC_HUMAN | Vinculin - Homo sapiens (Human) | 124292 | 5.5 | 755 | 26% | ↑ | actin binding | Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane; Peripheral membrane protein; |
11 | IGKC_HUMAN | Ig kappa chain C region - Homo sapiens (Human) | 11773 | 5.58 | 101 | 80% | ↑ | immuno | extracellular region |
12 | GNB1_HUMAN | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 - Homo sapiens (Human) | 38151 | 5.6 | 69 | 31% | ↓ | transducer |  |
17, 18, 20 | ACTB_HUMAN | Actin, cytoplasmic 1 | 42052 | 5.29 | 119 | 29% | ↑ | ATP binding | Cytoplasm; cytoskeleton. |