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Figure 1 | Proteome Science

Figure 1

From: Downregulated expression of HSP27 in human low-grade glioma tissues discovered by a quantitative proteomic analysis

Figure 1

Incorporation of Leu-d 3 in β-actin at different passages of H4 cells. A-D represented samples respectively from 1, 3, 5 and 7 passages of labeled H4 cells. The represented pairs of peptides "SYE L PDGQVITIGNER" from β-actin were analyzed at different time points by ESI-TOF/MS. The intensity ratio of pairs of isotope peaks (m/z, 897/895) was respectively about 65.78%, 94.92%, 97.23% and 97.67%. After seven passages, the incorporation of Leu-d3 was exceed 97.5%, which indicated that the labeling was complete.

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Proteome Science

ISSN: 1477-5956