Figure 5

Panel A and B indicate proteins no longer expressed after the 2^nd passage. Protein spots detected by image analysis (A) are indicated with circles, whereas the proteins assigned by PMF analysis are annotated with the abbreviation name; the magnified views and peptide mass spectrum of spots 2, 22, 34, 35, 36, 61, 67 and 75 are indicated (B). Panel C and D indicate proteins spots expressed at the 12^th passage. All detected spots are indicated with circles, whereas the identified protein spots are designed with the abbreviation name (C); the magnified views and peptide mass spectrum of spots 5, 16, 17,31 and 43 are indicated (D). Panel E and F indicate immunoblot analysis of the WJCs protein only expressed at the 2^nd and at the 12^th passages. 1-D gels probed with antibodies raised against KAD5, EIF31, PAI2, SHOT1, ERO1, PHA1 AND PHA2 (E). 2D blot analysis raised against ACTB and VIME at the 2^nd and 12^th passages (F).