Panel A and B indicate proteins no longer expressed after the 2nd passage. Protein spots detected by image analysis (A) are indicated with circles, whereas the proteins assigned by PMF analysis are annotated with the abbreviation name; the magnified views and peptide mass spectrum of spots 2, 22, 34, 35, 36, 61, 67 and 75 are indicated (B). Panel C and D indicate proteins spots expressed at the 12th passage. All detected spots are indicated with circles, whereas the identified protein spots are designed with the abbreviation name (C); the magnified views and peptide mass spectrum of spots 5, 16, 17,31 and 43 are indicated (D). Panel E and F indicate immunoblot analysis of the WJCs protein only expressed at the 2nd and at the 12th passages. 1-D gels probed with antibodies raised against KAD5, EIF31, PAI2, SHOT1, ERO1, PHA1 AND PHA2 (E). 2D blot analysis raised against ACTB and VIME at the 2nd and 12th passages (F).