Figure 7From: Proteome analysis of soybean leaves, hypocotyls and roots under salt stress Relative amount of mRNA for salt responsive genes in leaf, hypocotyl and root of soybean seedlings. Total RNA was extracted from 3- and 5-day-old hypocotyls and from 7-day-old leaves, hypocotyls and roots of soybean seedlings after 40 mM NaCl treatment. cDNA was synthesized by reverse transcription, and quantitative real-time PCR was performed using the cDNA products. An 18S rRNA cDNA clone was used as a template to produce a standard curve and the relative amount of mRNA was calculated by the ΔCt method. Values are the mean ± SE from 3 independent experiments and asterisks indicate significant (P < 0.05) differences between control (white columns) and NaCl treatments (black columns). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; S31, stem 31 kDa glycoprotein precursor; FK, putative fructokinase 2; L, leaf; H, hypocotyl; R, root; H3, 3-day-old hypocotyl and H5, 5-day-old hypocotyl.Back to article page