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Figure 7 | Proteome Science

Figure 7

From: Proteome analysis of soybean leaves, hypocotyls and roots under salt stress

Figure 7

Relative amount of mRNA for salt responsive genes in leaf, hypocotyl and root of soybean seedlings. Total RNA was extracted from 3- and 5-day-old hypocotyls and from 7-day-old leaves, hypocotyls and roots of soybean seedlings after 40 mM NaCl treatment. cDNA was synthesized by reverse transcription, and quantitative real-time PCR was performed using the cDNA products. An 18S rRNA cDNA clone was used as a template to produce a standard curve and the relative amount of mRNA was calculated by the ΔCt method. Values are the mean ± SE from 3 independent experiments and asterisks indicate significant (P < 0.05) differences between control (white columns) and NaCl treatments (black columns). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; S31, stem 31 kDa glycoprotein precursor; FK, putative fructokinase 2; L, leaf; H, hypocotyl; R, root; H3, 3-day-old hypocotyl and H5, 5-day-old hypocotyl.

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