Figure 1From: Proteomic analysis of prolactinoma cells by immuno-laser capture microdissection combined with online two-dimensional nano-scale liquid chromatography/mass spectrometry SDS-PAGE of PRL cells. PRL cells collected from LCM were lyzed with urea (7 M) and thiourea (2 M). Solubilized proteins were separated via SDS-PAGE and stained with Coomassie blue. The gel was cut into five sections based on molecular mass and each section was subjected to in-gel digestion with trypsin.Back to article page