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Figure 2 | Proteome Science

Figure 2

From: Highlights on the capacities of "Gel-based" proteomics

Figure 2

Impact of staining procedures on mass spectrometry compatibility using visible stains and fluorescent dyes. In these studies, the effects of protein spot properties were integrated to derive prediction of the MS results obtainable with the different dyes for all spots in the gels [24, 49]. 100 μg of total protein extracts from Arabidopsis thaliana were focused on pI 4-7 range and separated on gels covering the 15-150 kDa range. By comparison to sensitivity properties of dyes, these simulations enable a first estimation of the overall proteomic capacity of dyes. They argue for a clear advantage in using fluorescent dyes, particularly SR, which cumulates high sensitivity, acceptable identification success on gels loaded with low protein amount, and constant protein sequence coverage. Abbreviations used: colloidal Coomassie blue (CCB), silver nitrate (SN), Sypro Ruby (SR), Deep Purple (DP).

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