Figure 1

Detection of DHBV infected PDHs by indirect immunofluorescence. PDHs were prepared by liver perfusion and seeded on glass coverslips, and infected with DHBV concentrated from the supernatant of LMH-D2 by ultracentrifugation. PDHs were fixed with 4% polyoxymethylene. DHBV infected PDHs was detected by indirect immunofluorescence staining with mouse anti-DHBV preS mAb, and followed by goat anti-mouse IgG-FITC antibody. Then cells were stained with DAPI and visualized by confocal microscopy (Green, FITC; blue, DAPI). The symbol "p.i." represents DHBV post-infection PDHs.