Figure 2
From: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus
Comparison of 2-DE gel patterns between DHBV infected and uninfected PDHs. Proteins were extracted from DHBV infected and uninfected PDHs at 24, 72 and 120 h post-infection. Equal proteins (120 μg) were loaded and separated on the 24-cm pH 3-10 nonlinear IPG strips, followed by 12% SDS-PAGE gels visualized by silver staining. Differentially expressed protein spots on 2-DE gels were identified by MS/MS analysis and shown in gels with unique protein spot numbers. Gels of B, D, F and A, C, E represent 2-DE gels obtained from DHBV infected and uninfected PDHs at 24, 72 and 120 h post-infection, respectively. The numbers of unidentified spots are underlined and listed in Additional File 4, the numbers of up-regulated protein spots in infected samples are labeled in red and the numbers of down-regulated protein spots in blue color (A~F).