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Table 1 Differentially expressed proteins in DHBV-infected PDHs identified by MALDI-TOF/TOF

From: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus

Spot No.a

Protein

Species

Accession No.b

MOWSE Scorec

Tmw/Emwd

TpI/EpIe

Ratio: infected/uninfected

       

24h

72h

120h

 

Carbohydrate metabolism

        

1

Triosephosphate isomerase 1

Gallus gallus

gi|45382061

385

26.6/29

6.71/6.62

1.5

1

Nf

2

Triosephosphate isomerase

Meleagris gallopavo

gi|34221747

99

22.5/16

6.19/6.00

1.4

3

1

3

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Columba livia

gi|6016077

109

35.7/22

8.71/7.41

1

0.5

N

4

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Columba livia

gi|6016077

102

35.7/13

8.71/6.23

0.66

0.2

N

5

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Passer domesticus

gi|37698402

131

34.8/25

8.71/7.80

1

0.5

N

6

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Columba livia

gi|6016077

94

35.7/12

8.71/5.79

1

1

0.2

7

Phosphoglycerate kinase 1

Gallus gallus

gi|45384486

231

44.7/42

8.31/5.66

2.5

1.5

1

8

Phosphoglycerate mutase 1

Gallus gallus

gi|71895985

135

28.9/25

7.03/7.39

1.5

1.3

1

9

Alpha-enolase

Anas platyrhynchos

gi|213085

124

40.8/32

6.28/5.45

0.66

0.8

0.5

10

Alpha-enolase

Anas platyrhynchos

gi|119338

340

47.2/43

6.37/6.12

0.25

N

N

11

Aconitase 2, mitochondrial

Gallus gallus

gi|45383738

227

85.7/103

8.05/7.78

N

2

1.8

12

ATP5A1

Taeniopygia guttata

gi|91805309

380

21.5/23

6.4/5.85

1.2

0.9

2.3

13

Atp5a1 protein

Xenopus tropicalis

gi|71896075

146

59.8/24

9.13/6.47

1.3

0.56

1

14

Chain E, Chicken Cytochrome Bc1 Complex Inhibited By An Iodinated Analogue Of The Polyketide Crocacin-D

Xenopus tropicalis

gi|196049779

146

21.5/27

6.07/6.00

0.17

N

1

15

similar to ubiquinol--cytochrome c reductase

Gallus gallus

gi|50754375

72

52.7/24

6.58/5.45

1

1.2

0.5

 

Amino acid metabolism

        

16

unnamed protein product

Mus musculus

gi|74183518

118

35.8/28

6.84/6.80

1.5

1

1

17

similar to betaine homocysteine methyl transferase

Gallus gallus

gi|50755288

96

45/44

7.56/6.80

1.5

2

1.5

18

Pterin-4 alpha-carbinolamine dehydratase

Gallus gallus

gi|45382483

170

12.0/11

6.04/5.42

1

1.8

N

19

similar to Urocanase domain containing 1

Gallus gallus

gi|50754419

127

75.1/149

7.21/6.12

1

0.5

1.2

20

similar to Urocanase domain containing 1

Gallus gallus

gi|50754419

99

75.1/149

7.21/5.91

1

0.66

1.7

21

similar to 3-mercaptopyruvate sulfurtransferase

Gallus gallus

gi|50794693

144

33.2/32

5.66/5.80

0.75

0.25

N

22

Aldehyde dehydrogenase 4A1

Xenopus laevis

gi|148228402

99

61.7/58

8.22/7.18

N

3

1.2

 

Cytoskeletal/structural protein

        

23

Transgelin

Gallus gallus

gi|45382783

92

22.3/19

8.85/7.78

1

3

1.4

24

Vinculin

Gallus gallus

gi|45382123

82

116.9/116

6/6.12

N

3

1

25

Destrin

Gallus gallus

gi|45382979

97

18.5/18

7.52/6.60

1.5

1.33

2

26

Lamin A

Gallus gallus

gi|45384214

168

73.1/65

6.5/6.61

N

2

2

27

Lamin A

Gallus gallus

gi|45384214

74

73.1/66

6.5/6.40

N

1.5

2

28

similar to Myosin regulatory light chain 2,nonsarcomeric (Myosin RLC) isoform 1

Canis lupus familiaris

gi|73961895

241

20.5/15

4.62/3.80

1

1

A/Ng

29

similar to HSPC162(dynein, light chain)

Gallus gallus

gi|50758587

86

10.9/11

6.58/5.79

0.5

1

0.33

30

Collapsin response mediator protein-2B

Gallus gallus

gi|33340025

173

62.2/69

6.05/6.12

N

A/N

2

31

Cofilin-2

Gallus gallus

gi|17433708

74

18.6/14

7.66/5.20

0.33

1.2

0.67

32

Gelsolin

Gallus gallus

gi|45384386

95

85.8/14

5.93/6.19

N

0.25

1

33

similar to LIM protein

Danio rerio

gi|68371150

84

35.1/37

6.2/6.61

N/Ag

1.3

1

34

beta-actin

Labeo calbasu

gi|18034011

343

41.7/41

5.16/5.40

0.63

0.8

0.94

 

Stress response

        

35

similar to heat shock 70kDa protein 8 isoform 2 isoform 2

Canis lupus familiaris

gi|74012289

74

53.5/20

5.59/7.80

N

N

A/N

36

Chain A, T13g Mutant Of The ATPase Fragment Of Bovine Hsc70

Bos taurus

gi|6729825

237

41.9/29

6.63/5.72

0.7

0.33

1

37

Heat shock 70kDa protein 5 precursor

Gallus gallus

gi|45382769

526

72/96

5.12/5.00

N

A/N

1

38

Heat shock 70kDa protein 5 precursor

Gallus gallus

gi|45382769

471

72/96

5.12/5.00

N

A/N

1

39

Chaperonin containing TCP1, subunit 6A (zeta 1)

Gallus gallus

gi|57525300

211

57.6/65

6.36/6.60

1

2

1.3

40

58kDa glucose regulated protein precursor

Gallus gallus

gi|45383890

251

56.1/62

5.76/5.69

N

2

1

41

Mn superoxide dismutase

Cairina moschata

gi|184133036

235

12.9/24

6.39/7.00

1

1

1.71

42

similar to antioxidant protein isoform 2 (peroxiredoxin-3)

Sus scrofa

gi|194042134

256

26.6/25

6.79/6.80

1.5

1.43

1.5

43

LOC496089 protein

Xenopus laevis

gi|56269242

97

29.9/28

6.07/5.70

1

1

0.5

 

Other functions

        

44

Elongation factor 2

Oxyuranus scutellatus

gi|63146080

83

45.1/16

5.83/5.85

1

0.66

0.67

45

Elongation factor 2

Mus musculus

gi|192989

140

29.9/15

6.2/5.78

1

1

0.5

46

Cathepsin B

Gallus gallus

gi|46195455

73

37.6/25

5.74/5.08

1

1

1.5

47

ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

Taeniopygia guttata

gi|115391986

132

25.2/28

5.83/5.45

N

0.29

0.85

48

Ovotransferrin

Anas platyrhynchos

gi|3024757

144

75.6/91

6.19/6.60

N

1.5

1.3

49

Annexin A2

Gallus gallus

gi|45382533

148

38.6/31

6.92/5.45

0.33

1

0.5

50

Annexin A2

Gallus gallus

gi|45382533

204

38.6/10

6.92/5.78

0.5

N

0.2

51

Annexin A5

Anoplopoma fimbria

gi|229366222

75

34.9/12

5.28/5.00

0.5

1

0.86

  1. a) Spots numbers correspond to the numbers in Figure 2.
  2. b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database.
  3. c) Protein score was from MALDI-TOF/TOF identification. The proteins that had a statistically significant score great than 72 (p < 0.05) were considered identified.
  4. d) Theoretical/experimental molecular mass.
  5. e) Theoretical/experimental molecular pI.
  6. f) Not applicable, because the spots on the gels were too weak or non-detectable.
  7. g) A represents the spot on one of the gels was detectable, N represents the spot on one of the gels was too weak to detect.