Figure 1

Analysis of purified avian infectious bronchitis virus preparations. A: specific pathogen free (SPF) chick embryo-grown major particles H52 from 30%-40% sucrose density gradients, negatively stained with 2% potassium phosphotungstate, pH 6.5. B: SDS-PAGE separation of proteins in a purified H52 preparation. 8 μg of proteins were separated on an 5-17.5% polyacrylamide gel and stained with Coomassie blue. C: Western blotting of the purified H52 virions. Viral proteins were separated on 12% polyacrylamide gel and analyzed by western blot with chicken polyclonal antibody against infectious bronchitis virus (Massachusetts). The identified viral proteins are indicated. S: spike, N: nuclecapsid, M: mebrane, E: envelope.