Figure 4
From: Proteomic analysis of purified coronavirus infectious bronchitis virus particles
Confirmation of host proteins incorporated into infectious bronchitis virus particles with western blotting. 8 μg of purified virions from allantoic fluid (AF) (lane 1) and 15 μg of proteins extracted either from the normal 10-day-old specific pathogen free (SPF) chick embryo(CEL)(lane 2) or from the AF of the uninfected 10-day-old specific pathogen free (SPF) embryonated chicken eggs which were parallelly subjected to our standard density centrifugation procedure (lane 3) were subjected to western blot analysis with antibodies against the following proteins: (A) Actin, (B) Nucleocapsid (N) protein of the IBV, (C) Tubulin alpha-1, (D) Annexin A2, (E) Hsp90. Numbers to the left are molecular weight markers (M).