Figure 3From: Quantitative proteomic analysis by iTRAQ® for the identification of candidate biomarkers in ovarian cancer serum Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected.Back to article page