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Table 1 Summary of the number of proteins identified in serum after abundant protein depletion

From: Quantitative proteomic analysis by iTRAQ® for the identification of candidate biomarkers in ovarian cancer serum

  iTRAQ® Mass Tag Number of Proteins Identified Confidence Level Proteins Increased > 1.6 Fold
Depletion Experiment 114 115 116 117 ≥ 99% ≥ 95%  
MARS nd C1 N1 N1 75 86 5
IgY-12 Spin column C4 N3 C5 N6 114 138 9
IgY-12 HPLC set 1 N1 N4 C3 C6 88 107 5
IgY-12 HPLC set 2 N5 N6 C1 C4 109 134 4
IgY-12 HPLC set 3 N3 N2 C5 C2 87 113 2
  1. C1-C6 correspond to six pools of ovarian cancer serum (10 patients each).
  2. N1-N6 correspond to six pools of non-cancer control serum (10 patients each).
  3. nd, not done
  4. C/N ratio > 1.6 and N/N ratio between 0.8 and 1.2