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Table 1 Summary of the number of proteins identified in serum after abundant protein depletion

From: Quantitative proteomic analysis by iTRAQ® for the identification of candidate biomarkers in ovarian cancer serum

 

iTRAQ® Mass Tag

Number of Proteins Identified

Confidence Level

Proteins Increased > 1.6 Fold †

Depletion Experiment

114

115

116

117

≥ 99%

≥ 95%

 

MARS

nd

C1

N1

N1

75

86

5

IgY-12 Spin column

C4

N3

C5

N6

114

138

9

IgY-12 HPLC set 1

N1

N4

C3

C6

88

107

5

IgY-12 HPLC set 2

N5

N6

C1

C4

109

134

4

IgY-12 HPLC set 3

N3

N2

C5

C2

87

113

2

  1. C1-C6 correspond to six pools of ovarian cancer serum (10 patients each).
  2. N1-N6 correspond to six pools of non-cancer control serum (10 patients each).
  3. nd, not done
  4. †C/N ratio > 1.6 and N/N ratio between 0.8 and 1.2
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Proteome Science

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