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Figure 2 | Proteome Science

Figure 2

From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

Figure 2

Analysis of cell-free expression levels obtained using an E. coli S30 extract incubated under standard temperature conditions (30°C). Microarrays were scanned at 488 nm using a MDS GenePix Autoloader 4200AL, and fluorescence detected using a bandpass filter centered at 511 nm. Left Panel: The array surface is divided into three columns and five rows. High levels of fluorescence are observed only for sub-arrays A2, A3, and E2, representing the positive control (purified GST-GFP in the presence of Stabilguard), and the products from GST and MBP. Right Panel: The amounts of fluorescence were quantified from the scanned images, and the data presented in the order of sub-arrays A1, A2, A3, B1.....E1, E2, E3.

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Proteome Science

ISSN: 1477-5956