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Figure 4 | Proteome Science

Figure 4

From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

Figure 4

Comparison of cell-free expression levels obtained using an E. coli S30 extract, the wheat germ extract, and a hybrid system that combines the wheat germ and S30 extracts. Transcription/translation was done of the GFP control, and of the Arabidopsis PIL5 and PIF3 proteins. Microarrays were scanned at 488 nm using the MDS GenePix Autoloader 4200AL. Left Panel: Fluorescence signals are observed in the scanned image corresponding to cell-free GFP transcription/translation for all three extracts, but are only seen for the two Arabidopsis proteins using the hybrid extract. Right Panel: Quantification of the relative levels of expression by the three different extracts, for the three proteins. Abbreviations: PIF3, phytochrome-interacting factor 3; PIL5, PIF3-like 5 protein.

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