Figure 7From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts Comparison of the yields of fluorescent proteins obtained using the wheat germ, rabbit reticulocyte, and the hybrid cell-free expression systems on programmable arrays. Linear DNA (PCR products) for ankyrin-repeat protein, MBP, GFP, AXR3, and SHY2 was printed with anti-GFP to create programmable arrays. After protein expression with the three different systems, the slide was imaged and the fluorescence quantified.Back to article page