Figure 8From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts Characterization of the time courses of expression and final sizes of the recombinant proteins produced by the hybrid extract. A. Time Course. Cell-free expression reactions were set up within the wells of a microtiter plate, and fluorescence emission was quantified each hour over a four hour period using a Fluoroskan II fluorimeter. B. Protein Product Characterization. After cell-free expression, the target proteins were analyzed by western blotting, using peroxidase-labeled anti-GFP antibody for detection. Expected sizes in kDa: Ankyrin-repeat, 49.8; MBP, 70.2; GFP 27.3; CRY1, 106.4; AXR3, 55.9; SHY2, 52.1; RGA, 95.3; IAA9, 66.2; FYPP3, 62.6; PP2C, 60.5.Back to article page