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Table 2 Changes in protein expression between wild-type and SP-A-/- mice for control, 4 hr post infection and 24 hr post infection: percent changes with significance for all identified proteins corresponding to reference gels in Fig. 2

From: Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice

Gel No.

Protein

Functional Group

% Δ WT

to SP-A-/-

Baseline

% Δ WT to SP-A-/-

4 hr

% Δ WT

to SP-A-/-

24 hr

1

14-3-3-Zeta

DEF, RED, PMM

-34.97

16.24

-10.69

2

Adipsin (Complement factor D)

DEF, PMM

-63.51

5.95

4.76

3

Albumin

RED

4.82

1.56

12.73

4

Aldehyde dehydrogenase AHD-M1

RED

-82.62

37.46

29.21

5

Aldehyde dehydrogenase II

RED

135.85

14.63

31.75

6

Aldehyde dehydrogenase, Dimeric NADP-preferring (EC 1.2.1.5) (ALDH class 3)

RED

58.04

40.10

38.77

7

Alpha-1-antitrypsin 1-1 precursor (Serine protease inhibitor 1-1)

DEF, PMM

-30.98

15.12

16.63

8

Alpha-1-antitrypsin 1-6 precursor (Serine protease inhibitor 1-6) (Alpha-1 protease inhibitor)

DEF, PMM

-12.72

-9.38

-12.84

9

Alpha-fetoprotein

 

-16.1

24.43

-11.61

10

Annexin A1 (Annexin I) (Lipocortin I) (Calpactin II) (Chromobindin-9) (P35)

DEF

-201.08

72.45

4.89

11

Annexin A3

 

21.76

-7.73

1.22

12

Annexin A4

 

13.78

14.08

3.38

13

Annexin A5

DEF

-381.16

-66.73

-130.01

14

Apolipoprotein A-1

DEF, RED

-23.3

-89.53

93.80

15

Beta-2-microglobulin

DEF

68.18

-59.79

-55.69

16

Beta-actin

 

-14.27

32.95

13.93

17

Beta-actin (putative, AA 27-375) (alpha-actin)

 

4.84

52.39

9.19

18

Carbonyl reductase 2

RED

-184.18

29.93

0.81

19

Cell specific 10K protein (uteroglobin - mouse) (Clara cell 10K protein) (CC10) (CC16)

DEF, RED

-73.76

12.90

64.31

20

Ceruloplasmin isoforms

RED

22.02

-9.13

-19.71

21

Chain A, Crystal structure of novel mammalian lectin Ym1-suggests a saccharide binding site

DEF

-2.98

28.89

13.35

22

Chain B, Chimeric human mouse carbonmonoxyhemoglobin (Human zeta, Mouse beta 2)

 

-5.61

8.67

33.51

23

Chia protein

DEF

5.35

31.40

17.25

24

Coiled-coil domain containing 122

 

-183.85

21.31

-6.43

25

Complement component 3

DEF

-12.5

40.66

8.39

26

Complement component C5

DEF

-22.76

21.75

5.19

27

Contrapsin (Serine protease inhibitor A3K)

PMM

7.77

-8.52

-8.74

28

Creatine kinase M-type (EC.2.7.3.2) (Creatine kinase M chain) (M-CK)

 

-57.22

28.34

-22.83

29

Cytosolic malate dehydrogenase

RED

-3.05

7.03

1.31

30

(Similar to) Ferritin light chain 1 (Ferritin L, subunit 1)

DEF, RED

-87.39

-14.84

-3.35

31

Gamma-actin

 

-20.73

31.85

20.75

32

Gelsolin precursor (Actin-depolymerizing factor) (ADF) (Brevin)

DEF, RED, PMM

30.02

-3.09

-2.90

33

Glutathione S-transferase, alpha 3

DEF, RED

-193.11

21.05

19.07

34

Glutathione S-transferase, alpha 4

DEF, RED

-339.42

64.33

38.47

35

Glutathione S-transferase, mu 1

DEF, RED

-101.12

45.92

35.09

36

Glutathione S-transferase, omega 1

DEF, RED

-132.89

48.91

15.84

37

(Similar to) Glutathione S-transferase, Ya chain (GST class-alpha) (Ya1)

DEF, RED

-318.76

56.63

44.05

38

Haptoglobin

DEF, RED

-14.69

16.92

2.41

39

Hemoglobin subunit alpha (Hemoglobin alpha chain)(Alpha-globin)

 

-6.6

-32.60

132.59

40

Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Cytosolic NADP-Isocitr)

RED

-21.59

17.31

1.14

41

Keratin complex 1, acidic, gene 10

 

16.11

20.63

-30.55

42

(Similar to) Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10)

 

-43.31

26.68

-5.45

43

Kpnb1 protein b

 

-55.63

53.13

34.52

44

Lactate dehydrogenase 2, B chain

DEF, RED

28.46

-4.34

-3.26

45

Lysozyme 2

DEF, RED

129.29

-63.56

-133.43

46

Murinoglobulin-1 precursor (MuG1)

DEF, PMM

-14.21

-16.51

-6.93

47

Myosin heavy chain IIB

 

-21.97

6.81

-15.67

48

Peroxiredoxin 1

RED

-83.18

24.93

11.30

49

Peroxiredoxin 6

DEF, RED

-54.85

56.63

42.36

50

Pregnancy zone protein

DEF, PMM

-1.56

2.46

-7.38

51

Prothrombin precursor (Ec 3.4.21.5) (Coagulation factor II)

DEF, PMM

-59.43

6.39

5.13

52

Pulmonary surfactant associated protein A precursor (SP-A) (PSP-A) (PSAP)

DEF, RED

ND

ND

ND

53

Rho GDP dissociation inhibitor (GDI) alpha

 

-115.4

-22.62

-42.11

54

SEC14-like 3

PMM

-33.59

30.46

9.92

55

Selenium binding protein 1

DEF, RED

9.26

-4.10

-3.32

56

Selenium binding protein 2

DEF, RED

27.17

-2.40

-3.86

57

Serine (or cysteine) proteinase inhibitor, clade A, member 1e

PMM

7.7

-18.15

-11.72

58

Toll-like receptor 13 precursor

DEF

18.41

-17.04

-4.57

59

Transferrin

DEF, RED, PMM

8.65

-17.12

-0.37

60

Tyrosine-3-monooxygenase/tryptophan 5-monooxynase activation protein, Epsilon polypeptide

PMM

-183.11

18.41

-7.70

  1. Changes (% Δ) in BAL protein expression from wild-type (WT) to SP-A-/- mice are shown for untreated (baseline) animals, 4 hours post-infection and 24 hours post-infection. Gel numbers (see Fig. 2), protein names, functional groups and percent change (% Δ) are listed. Abbreviations for functional groups: DEF, host defense; PMM, protein modification and metabolism; RED, redox regulation. Bolded numbers indicate changes that were significant (p < 0.05 by t-test). Values for SP-A, which is absent in SP-A-/- mice, are listed as not determined (ND).