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Figure 4 | Proteome Science

Figure 4

From: A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS

Figure 4

Identification of proteins from rubber latex via MALDI TOF/TOF MS. The 2 protein spots (spots 1 and 1 from total rubber latex) were excised and digested with trypsin, and then the collected peptides were analyzed using an Autoflex MALDI-TOF/TOF mass spectrometer. The annotated PMF spectral peaks showed the intensities of different peptides (A for spot 1 and D for spot 2). The sequences marked with colour and dark letters represent the matched and unmatched peptides, respectively. The matched peptides marked with under-lines were further identified with MALDI TOF-TOF MS/MS and the annotated spectrum of CID (collision induced dissociation) was presented (B and C from spot 1; E and F from spot 2). Database searching with Mascot software against NCBInr database identified the 2 proteins as SRPP (spot 1) and REF (spot 2) from rubber tree respectively.

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ISSN: 1477-5956