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Table 2 Proteins identified from rubber latex via MALDI TOF/TOF MS/MS

From: A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS

No.a Acc.b Protein name (TOF/TOF sequence)c Exper. p I/kDad Thero. p I/kDae MP/TPf SC (%)g Scores (PFF)h Plant species
1 14423933 Small rubber particle protein
(K.QVSAQTYSVAQDAPR.I)
(R.IVLDVASSVFNTGVQEGAK.A) 		4.71/25 4.80/22 13/62 85
(7)
(9) 		94
(91)
(124) 		H. brasiliensis
2 132270 Rubber elongation factor
(K.NVAVPLYNR.F)
(K.DKSGPLQPGVDIIEGPVK.N) 		4.95/12 5.04/15 10/33 78
(6)
(13) 		125
(59)
(80) 		H. brasiliensis
3 10946499 beta-1,3-glucanase
(R.YIAVGNEISPVNR.G) 		7.02/34 9.46/35 15/48 47
(3) 		103
(50) 		H. brasiliensis
4 3288200 latex allergen
(K.SLDCEDYYLR.I) (R.DTYDPIHSIGPIYDGEYLR.E) 		4.87/44 5.00/43 22/42 68
(2)
(4) 		214
(71) (122) 		H. brasiliensis
5 132270 Rubber elongation factor
(K.DKSGPLQPGVDIIEGPVK.N) 		5.08/15 5.04/15 10/33 60
(13) 		126
(76) 		H. brasiliensis
6 14423933 Small rubber particle protein
(K.QVSAQTYSVAQDAPR.I) 		4.88/24 4.80/22 14/37 87
(7) 		161
(91) 		H. brasiliensis
7 14423933 Small rubber particle protein
(K.ENENFQQEANEQEEK.L) 		4.65/23 4.80/22 13/62 85
(12) 		94
(96) 		H. brasiliensis
8 6707018 latex allergen
(R.DTYDPIHSIGPIYDGEYLR.E) 		5.01/43 5.00/43 15/52 45
(4) 		139
(104) 		H. brasiliensis
9 58013197 Actin
(K.NYELPDGQVITIGAER.F) 		5.12/42 5.31/42 20/60 53
(4) 		125
(68) 		I. tinctoria
10 10946499 beta-1,3-glucanase
(R.NIHDAIR.S)
(R.YIAVGNEISPVNR.G) 		6.87/34 9.46/35 20/64 57
(2)
(3) 		168
(106)
(60) 		H. brasiliensis
11 10946499 beta-1,3-glucanase
(R.IYDPNQAVLEALR.G) 		6.89/33 9.46/35 19/51 68
(3) 		173
(107) 		H. brasiliensis
12 157831407 Chain A, Hevamine
(K.VYLTAAPQCPFPDR.Y) 		7.08/30 8.44/30 7/10 50
(4) 		99
(54) 		H. brasiliensis
13 124365253 Chain A, Hydroxynitrile Lyase
(K.RPFFTK.E)
(K.VTALDLAASGVDPR.Q) 		6.22/11 5.15/30 9/24 42
(2)
(5) 		93
(92)
(86) 		H. brasiliensis
  1. aAssigned spot number as indicated in Fig 2 and Fig 3.
  2. bDatabase accession numbers according to NCBInr.
  3. cThe name of proteins identified by MALDI TOF MS, and the peptide sequences produced by TOF/TOF MS/MS were presented in the bracket.
  4. d, eExperimental (d) and theoretical (e) mass (kDa) and pI of the identified proteins.
  5. fNumber of matched peptides with PMF data and the total searched peptides.
  6. gThe amino acid sequence coverage for the identified proteins.
  7. hMascot score against the database NCBInr for PMF and PFF (in the bracket).
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