Skip to main content

Table 2 Proteins identified from rubber latex via MALDI TOF/TOF MS/MS

From: A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS

No.a

Acc.b

Protein name (TOF/TOF sequence)c

Exper. p I/kDad

Thero. p I/kDae

MP/TPf

SC (%)g

Scores (PFF)h

Plant species

1

14423933

Small rubber particle protein

(K.QVSAQTYSVAQDAPR.I)

(R.IVLDVASSVFNTGVQEGAK.A)

4.71/25

4.80/22

13/62

85

(7)

(9)

94

(91)

(124)

H. brasiliensis

2

132270

Rubber elongation factor

(K.NVAVPLYNR.F)

(K.DKSGPLQPGVDIIEGPVK.N)

4.95/12

5.04/15

10/33

78

(6)

(13)

125

(59)

(80)

H. brasiliensis

3

10946499

beta-1,3-glucanase

(R.YIAVGNEISPVNR.G)

7.02/34

9.46/35

15/48

47

(3)

103

(50)

H. brasiliensis

4

3288200

latex allergen

(K.SLDCEDYYLR.I) (R.DTYDPIHSIGPIYDGEYLR.E)

4.87/44

5.00/43

22/42

68

(2)

(4)

214

(71) (122)

H. brasiliensis

5

132270

Rubber elongation factor

(K.DKSGPLQPGVDIIEGPVK.N)

5.08/15

5.04/15

10/33

60

(13)

126

(76)

H. brasiliensis

6

14423933

Small rubber particle protein

(K.QVSAQTYSVAQDAPR.I)

4.88/24

4.80/22

14/37

87

(7)

161

(91)

H. brasiliensis

7

14423933

Small rubber particle protein

(K.ENENFQQEANEQEEK.L)

4.65/23

4.80/22

13/62

85

(12)

94

(96)

H. brasiliensis

8

6707018

latex allergen

(R.DTYDPIHSIGPIYDGEYLR.E)

5.01/43

5.00/43

15/52

45

(4)

139

(104)

H. brasiliensis

9

58013197

Actin

(K.NYELPDGQVITIGAER.F)

5.12/42

5.31/42

20/60

53

(4)

125

(68)

I. tinctoria

10

10946499

beta-1,3-glucanase

(R.NIHDAIR.S)

(R.YIAVGNEISPVNR.G)

6.87/34

9.46/35

20/64

57

(2)

(3)

168

(106)

(60)

H. brasiliensis

11

10946499

beta-1,3-glucanase

(R.IYDPNQAVLEALR.G)

6.89/33

9.46/35

19/51

68

(3)

173

(107)

H. brasiliensis

12

157831407

Chain A, Hevamine

(K.VYLTAAPQCPFPDR.Y)

7.08/30

8.44/30

7/10

50

(4)

99

(54)

H. brasiliensis

13

124365253

Chain A, Hydroxynitrile Lyase

(K.RPFFTK.E)

(K.VTALDLAASGVDPR.Q)

6.22/11

5.15/30

9/24

42

(2)

(5)

93

(92)

(86)

H. brasiliensis

  1. aAssigned spot number as indicated in Fig 2 and Fig 3.
  2. bDatabase accession numbers according to NCBInr.
  3. cThe name of proteins identified by MALDI TOF MS, and the peptide sequences produced by TOF/TOF MS/MS were presented in the bracket.
  4. d, eExperimental (d) and theoretical (e) mass (kDa) and pI of the identified proteins.
  5. fNumber of matched peptides with PMF data and the total searched peptides.
  6. gThe amino acid sequence coverage for the identified proteins.
  7. hMascot score against the database NCBInr for PMF and PFF (in the bracket).
Back to article page

Proteome Science

ISSN: 1477-5956