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Figure 2 | Proteome Science

Figure 2

From: Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification

Figure 2

Optimizing number of amplification cycles. Twelve replicate spots of a colon cancer cell line containing 35 fg pERK were printed on nitrocellulose-coated slides. Detection of pERK was performed by standard near-infrared detection and AMSA comprising different rounds of amplification. Median fluorescence (black) and signal-to-noise ratios (red) were calculated. The blue line summarizes the results for AMSA optimization.

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