Figure 4
From: Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification
Comparing the sensitivity of standard NIR detection and AMSA for JNK spike-in. Dilution series of JNK (0.27 fg - 0.56 pg JNK per spot) in a colon cancer cell line lysate. Each dilution step was printed in 12 replicate spots. Samples were analyzed using standard NIR detection and AMSA on two-pad slides in parallel. Slides were scanned at intensity 5 on the Odyssey instrument. Vertical red lines indicate the calculated detection limits.