2-D electrophoresis identified five cardiac mitochondrial proteins that are modified following ischemia and reperfusion. (A) Western blots for GAPDH and mtCK to assess the purity of the mitochondrial preparation from aerobic hearts. (B) Representative 2-D electrophoresis of heart mitochondria indicating spots picked for identification. (C) Quantification of spots and representative gels. Values represent the mean ± SEM of 3 hearts per group. * represents P < 0.05, significantly different from aerobic value.