2-D electrophoresis identified two forms of the βE1 of pyruvate dehydrogenase (PDH). (A) Representative 2-D electrophoresis of heart mitochondria indicating spots identified as βE1 subunit of PDH. (B) Quantification of spots identified as the βE1 of PDH peptides and representative gels. Values represent the mean ± SEM of 3 hearts per group. * represents P < 0.05, significantly different from aerobic value.