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Table 1 Mass Spectrometry identification of protein spots using the Mascot Search Engine.

From: Novel O-palmitolylated beta-E1 subunit of pyruvate dehydrogenase is phosphorylated during ischemia/reperfusion injury

Protein

Probability Based Mowse Score*

Peptides

Sequence

Identification and AC** (#)

Spot (#)

Threshold

Observed

Matched

Not matched

Coverage

 
 

(P < 0.05)

Score

(n)

(n)

(%)

 
      

NADH-ubiquinone

1

58

141

17

41

44

oxidoreductase (24 kDa subunit),

      

P19234

      

Pyruvate dehydrogenase

2

32

108

3

31

10

(β E1 subunit)

      

P49432

      

ATP synthase

3

58

127

13

40

34

(D subunit)

      

P31399

4

58

127

13

18

34

Long-chain acyl-CoA dehydrogenase

      

P15650

      

ATP synthase

5

58

87

12

28

41

(α subunit)

      

P15999

  1. * -10 log(P) where P is the probability that the observed match is a random event.
  2. **UniProtKB/Swiss-Prot primary accession number