Figure 2

Western blot analysis of hemocyanin protein expression levels in hemocytes of Vibrio harveyi -challenged Penaeus monodon. (A) The expression of hemocyanin protein in the hemocytes of control (saline-injected) and V. harveyi-challenged shrimps at 0 and 48 hpi, as assayed by western blot analysis using an antibody specific to hemocyanin. The protein expression level at each time point was then normalized to that of phosphopyruvate hydratase. (B) Fold change of hemocyanin expression level after V. harveyi infection at 0 (0 h_I) and 48 h (48 h_I) compared to that of control shrimp (0 h_N) was shown. The data is shown as the mean ± 1S.D. and is derived from 3 repeats. Means with different letters are significantly different (p < 0.05).