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Table 1 Thirty nine selected 2-DE spots (proteins) from the hemocytes of Vibrio harveyi-challenged Penaeus monodon, resolved by 2-DE and identified by LC-nano ESI-MS/MS.

From: Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection

Spot no.

Predicted MW (Da)

Predicted pI

Protein hit

Mowse

Score/no. of match peptides

Accession no.

Fold change*

      

24 h

48 h

Up-regulated protein spots

    

33

78700

6.05

prophenoloxidase 2 [Litopenaeus vannamei]

71/2

ABQ45957

U

U

30

41822

5.11

actin 2 [Penaeus monodon]

240/7

AAC78682

U

U

3

74934

5.27

hemocyanin [Litopenaeus vannamei]

99/4

CAA57880

-1.35

+6.88

16

  

unknown

  

-2.92

+4.11

35

40087

6.05

arginine kinase [Penaeus monodon]

485/13

AAO15713

+1.85

+4.09

29

40087

6.05

arginine kinase [Penaeus monodon]

704/32

AAO15713

+2.33

+3.16

2

17142

6.74

twinstar [Drosophila melanogaster]

150/8

NP_477034

+1.88

+1.20

37

49283

4.92

tubulin alpha chain [Oncorhynchus keta]

94/3

P30436

-1.38

+1.88

4

52810

5.08

serine proteinase-like protein [Penaeus monodon]

119/5

ABD62888

+1.69

-1.25

32

37134

6.73

transaldolase [Bombyx mori]

110/1

NP_001040544

+1.60

+1.58

Down-regulated protein spots

    

20

32431

5.04

alpha-2-macroglobulin [Penaeus monodon]

59/1

AAX24130

D

D

9

16671

4.04

calmodulin [Patinopecten sp.]

54/3

0711223A

D

-13.89

19

29054

4.65

14-3-3 protein epsilon [Danio rerio]

114/5

NP_997770

D

+1.12

12

  

unknown

  

-1.08

-3.75

14

96846

4.73

karyopherin (importin) beta [Nematostella vectensis]

109/3

XP_001636221

-8.14

-3.21

8

  

unknown

  

-3.42

-2.70

36

78700

6.05

prophenoloxidase 2 [Litopenaeus vannamei]

119/3

ABQ45957

-3.49

-2.64

11

78700

6.05

prophenoloxidase 2 [Litopenaeus vannamei]

127/3

ABQ45957

-2.31

-2.87

7

  

unknown

  

-1.59

-2.73

13

24283

6.52

GTP-binding nuclear protein Ran (GTPase Ran) (Ras-like protein TC4) [Brugia malayi]

104/2

P38542

-1.52

-2.75

27

  

Unknown

  

-1.35

-2.39

6

52810

5.08

serine proteinase-like protein [Penaeus monodon]

84/2

ABD62888

-1.37

-2.42

10

78426

5.83

Prophenoloxidase-1 [Penaeus monodon]

973/20

AAM77689

-1.88

-2.32

31

56040

5.10

ATP synthase subunit beta, mitochondrial precursor [Hemicentrotus pulcherrimus]

411/7

Q25117

-2.06

-1.17

5

52810

5.08

serine proteinase-like protein [Penaeus monodon]

119/5

ABD62888

-1.92

-1.48

15

83278

4.89

heat shock protein 90 [Metapenaeus ensis]

484/17

ABR66910

-1.17

-1.59

25

  

Unknown

  

-1.40

-1.62

Constant protein spots

    

38

28203

5.89

thymosin beta-11 [Penaeus monodon]

419/10

BI018085

+1.41

+0.98

34

46851

4.41

calreticulin [Gallus gallus]

82/3

AAS49610

-1.17

+1.48

28

31358

5.42

cytosolic manganese superoxide dismutase [Penaeus monodon]

421/12

AAW50395

+1.18

-1.50

26

40087

6.05

arginine kinase [Penaeus monodon]

103/3

AAO15713

+1.03

+1.16

24

47235

6.18

phosphopyruvatehydratase [Penaeus monodon]

452/9

AAC78141

+1.06

+1.01

17

32574

4.78

hypothetical protein LOC550536 [Danio rerio]

58/3

NP_001017838

+1.24

+1.12

18

32420

4.70

tropomyosin [Locusta migratoria]

305/17

P31816

+1.47

+1.06

1

41841

5.30

beta-actin [Litopenaeus vannamei]

1083/80

AAG16253

+1.46

+1.32

39

  

Unknown

  

+1.12

+1.21

21

  

Unknown

  

-1.07

-1.36

22

  

Unknown

  

+1.21

+1.14

23

  

Unknown

  

-1.24

+1.19

  1. * indicates the decrease in the spot intensity at a given time point after V. harveyi infection compared to that of normal shrimp.
  2. + indicates the increase in the spot intensity at a given time point after V. harveyi infection compared to that of normal shrimp.
  3. U indicates the up-regulated protein spot detected only in the protein extracts of infected animals.
  4. D indicates the down-regulated protein spot detected only in the protein extracts of normal animals.