Table 2 List of primers and optimal PCR amplification conditions used for the quantitative Real-time RT-PCR
Gene | Primer name (Sequence (5'-3')) | Final concentration (nM) | Temperature (°C)/time (sec) | ||
|---|---|---|---|---|---|
| Â | Â | Â | Denaturation | Annealing | Elongation |
Alpha-2-macroglobulin | A2M_F (CCTCATATCCGGCTTCATCC) | 100 | 95/10 | 60/20 | 72/10 |
| Â | A2M_R (CCGTGAACTCCTCGATGTAG) | 100 | Â | Â | Â |
14-3-3 protein epsilon | 14-3-3_F (GTATCTTGCCGAGACCGCCACT) | 200 | 95/10 | 63/15 | 72/20 |
| Â | 14-3-3_R (CAATGTCGCTGGCTGCCTTGT) | 200 | Â | Â | Â |
Hemocyanin | Hemocyanin_F [46] (AAGTGCTCGGAATCTTCGGTAA) | 200 | 95/10 | 60/15 | 72/10 |
| Â | Hemocyanin_R [46] (CCTGCCTCGATCTTTGCAA) | 200 | Â | Â | Â |
Beta actin | Beta_F (GAACCTCTCGTTGCCGATGGTG) | 200 | 95/30 | 60/30 | 72/45 |
| Â | Beta_R (GAAGCTGTGCTACGTGGCTCTG) | 200 | Â | Â | Â |