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Figure 1 | Proteome Science

Figure 1

From: Proteomic analysis of proteins expressing in regions of rat brain by a combination of SDS-PAGE with nano-liquid chromatography-quadrupole-time of flight tandem mass spectrometry

Figure 1

Flow diagram of the experimental design. Rat brains were divided into six regions: thalamus, hippocampus, frontal cortex, parietal cortex, occipital cortex, and amygdala. The divided samples were lysed in lysis buffer containing SDS, and subjected to SDS-PAGE with Coomassie Brilliant Blue staining. The gel lane was divided into 24 slices, and the slices were pre-treated by in-gel trypsin digestion. The amino acid sequences of all detected proteins were determined by nano-LC-Q-TOF-MS/MS.

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Proteome Science

ISSN: 1477-5956