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Figure 5 | Proteome Science

Figure 5

From: Proteomic analysis of proteins expressing in regions of rat brain by a combination of SDS-PAGE with nano-liquid chromatography-quadrupole-time of flight tandem mass spectrometry

Figure 5

Immunoblotting analysis of proteins identified in six regions of rat brain. Rat brains were divided into six regions (thalamus, hippocampus, frontal cortex, parietal cortex, occipital cortex, and amygdala), and were extracted with lysis buffer. The samples (2.0 μg protein) were subjected to immunoblotting with anti-GFAP antibody (A), anti-GAT 3 antibody (B), anti-Septin 5 antibody (C), anti-HSP 90 antibody (D), anti-synaptotagmin antibody (E), and anti-HSP 70 antibody (F). Immunoblotting data using the indicated antibodies are shown in the upper panels; Mascot scores of the proteins determined by Q-TOF-MS/MS are shown in the lower panels. The immunoblotting data are shown as representative blots obtained in independent experiments from 3 rats. Data represent the means ± SE. Statistical significance was determined by ANOVA followed by Bonferroni's test. *1 p < 0.05 vs Hip, Fro, Par, Occ, and Amy, *2 p < 0.05 vs Amy, *3 p < 0.05 Amy and Hip.

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ISSN: 1477-5956