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Table 3 Rhipicephalus spp. tick differentially expressed proteins identified by MALDI-TOF MS and LC-MS/MS after saturation 2-D DIGE analysis.

From: Application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples

Spot

number a)

Accession number b)

Protein ID b)

Mw/pI c)

Protein Score d)

Number

of matched peptides

Sequence coverage (%)

Average -fold change e)

Tick/Pathogen

1

3319897

Albumin (Canis familiaris)

67.8/5.3

123

11

19.0

+ 5.7

R. sanguineus/E. canis

       

+ 7.8

R. bursa/T. annulata

2

3319897

Albumin (Canis familiaris)

67.8/5.3

127

11

29.6

+24.6

R. sanguineus/E. canis

       

+17.5

R. bursa/T. annulata

3

3319897

Albumin (Canis familiaris)

67.8/5.3

125

13

30.1

+38.8

R. sanguineus/E. canis

       

+16.9

R. bursa/T. annulata

4

3319897

Albumin (Canis familiaris)

67.8/5.3

85

9

22.2

+20.2

R. sanguineusy/E. canis

       

+18.0

R. bursa/T. annulata

5

3319897

Albumin (Canis familiaris)

67.8/5.3

106

12

26.5

+12.8

R. sanguineus/E. canis

       

+26.9

R. bursa/T. annulata

6

3319897

Albumin (Canis familiaris)

67.8/5.3

128

11

28.0

+ 9.8

R. sanguineus/E. canis

       

+28.6

R. bursa/T. annulata

7

-

Not identified

-

-

-

-

-5.1

R. bursa/T. annulata

       

-5.0

R. turanicus/A. ovis

8

5164373

Pre-pro serum albumin (Ovis aries)

69.2/5.8

IT

41

31.3

-5.5

R. bursa/T. annulata

       

-5.3

R. turanicus/A. ovis

9

193085052

Albumin precursor (Capra hircus)

66.3/5.6

88

17

27.6

-5.2

R. bursa/T. annulata

       

-5.1

R. turanicus/A. ovis

10

241157545

Actin, putative (Ixodes scapularis)

37.6/5.4

121

8

31.3

-7.9

R. sanguineus/R. conorii

       

-5.6

R. bursa/T. annulata

       

-5.1

R. turanicus/A. ovis

11

59894747

Actin (Ixodes ricinus)

41.5/5.6

121

11

42.0

- 5.5

R. sanguineus/R. conorii

       

+6.6

R. sanguineus/E. canis

       

- 5.9

R. bursa/T. annulata

12

-

Not identified

-

-

-

-

- 5.1

R. sanguineus/R. conorii

       

- 6.3

R. bursa/T. annulata

       

- 5.7

R. turanicus/A. ovis

13

258499

Haptoglobin heavy chain, HpH chain (Canis familiaris)

27.3/5.8

104

7

37.1

+36.7

+28.3

+12.4

R. sanguineus/R. conorii

R. sanguineus/E. canis

R. bursa/T. annulata

14

215497327

Enolase (Ixodes scapularis)

21.5/8.9

IT

4

18.1

- 8.2

R. sanguineus/R. conorii

       

- 6.7

R. bursa/T. annulata

       

- 5.5

R. turanicus/A. ovis

15

67083997

Guanine nucleotide-binding protein (Ixodes scapularis)

36.0/7.1

IT

2

9.4

-28.7

- 7.9

- 5.1

R. sanguineus/R. conorii

R. bursa/T. annulata

R. turanicus/A. ovis

16

157399341

Unknown larval protein (Rhipicephalus annulatus)

19.1/6.16

IT

3

17.5

-25.6

-7.3

+18.5

R. sanguineus/R. conorii

R. bursa/T. annulata

R. turanicus/A. ovis

17

157399341

Unknown larval protein (Rhipicephalus annulatus)

19.1/6.16

IT

5

11.1

- 12.4

+17.8

R. bursa/T. annulata

R. turanicus/A. ovis

18

44887976

Full hemoglobin subunit beta (Chrysocyon brachyurus)

16.1/8.0

112

10

59.6

+38.6

R. sanguineus/R. conorii

       

+ 9.5

R. sanguineus/E. canis

       

+17.0

R. bursa/T. annulata

19 (mix)

116618139

Conjugal transfer protein, ATPase (Leuconostoc mesenteroides)

95.8/5.8

86

16

17.1

+62.4

R. bursa/T. annulata

 

44887976

Full hemoglobin subunit beta (Chrysocyon brachyurus)

16.1/8.0

75

8

56.2

+13.1

R. sanguineus/E. canis

       

+38.6

R. sanguineus/R. conorii

20

44888810

Full hemoglobin subunit alpha (Canis familiaris)

15.3/8.0

111

7

63.8

+66.7

+ 7.0

+35.9

R. sanguineus/R. conorii

R. sanguineus/E. canis

R. bursa/T. annulata

21

44888810

Full hemoglobin subunit alpha (Canis familiaris)

15.3/8.0

IT

7

29.3

+41.5

R. sanguineus/R. conorii

       

+30.7

R. bursa/T. annulata

  1. a) Spot numbers refer to the 2 D gel proteins of interest that were analyzed by mass spectrometry (Figure 3).
  2. b) Accession number and protein identity are listed according to the NCBInr database for the best match.
  3. c) Abbreviations: Mw, molecular weight (kDa); pI, isoelectrical point.
  4. d) Protein score is-10*Log(P), where P is the probability that the observed match is a random event, it is based on NCBInr database using MASCOT Peptide Mass Fingerprinting searching program. When IT appears, it means that these proteins were identified by LC-MS/MS.
  5. e) + indicates an increase in protein expression and - indicates a decrease in protein expression in infected ticks.
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