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Table 1 Summary of the proteins secreted by D. seriata.

From: Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

Spot

Acc. number

Protein identity a

PM/Seq cov./score b

Best peptides (PRO-BLAST)

Identified by

Experimental MW/pI

Theoretical MW/pI

Organism

Signal P

S1

gi|256724272

Hypothetical protein

2/20%/83

 

MASCOT

13.2/7.6

13.5/9.6

Nectria haematococca

NNc

S2

gi|255935271

Pc13g02210

2/14%/79

KIIYPAYTDK

ARIKGDVVKPDKSYAPKAIP

PRO-BLAST

13.2/7.8

14.5/3.7

Penicillium chrysogenum

0.999

S3

gi|256724272

Hypothetical protein

2/20%/82

 

MASCOT

13.4/5.6

13.5/9.6

Nectria haematococca

NN

S4

gi|27924024

glucosidase

4/11%/97

GFNIGATNADGSC*Kd

EGGHVSISAVSRSEDLYRGDSDASK

PRO-BLAST

30.2/4.7

33.4/9.3

Fusarium sporotrichioides

0.999

S5

gi|256732553

Predicted protein (necrosis and ethylene inducing peptide 2 precursor)

2/7%/97

GELHNDAFALFYAWYFAPG

AVLAHDAVVGFAETVPFVGVWHAHK

PRO-BLAST

35.7/4.7

24.69/7.75

Nectria haematococca

0.999

S6

gi|239931549

Secreted protein

2/9%/105

SGASSWTAAR

SSSFPHWLTLSGSC*NTR

PRO-BLAST

26.3/4.2

23.03/8.35

Streptomyces ghanaensis

0.989

S7

gi|189192036

Hypothetical protein

3/18%/94

 

MASCOT

19.4/5.1

22.5/7.6

Pyrenophora tritici-repentis

0.999

S8

gi|256726602

Hypothetical protein (necrosis-and ethylene-inducing protein 1)

3/8%/77

FGLFYAWYFPK

ALSPHDSLTKDSYVR

PRO-BLAST

33.8/5.5

26.99/9.2

Nectria haematococca

1.000

S9

gi|156061869

Hypothetical protein (necrosis-and ethylene-inducing protein 1)

6/21%/77

 

MASCOT

26.0/5.2

26.9/6.6

Sclerotinia sclerotiorum

0.994

S10

gi|189196154

NADP-specific glutamate DH

9/31%/302

 

MASCOT

48.0/6.9

49.1/5.9

Pyrenophora tritici-repentis

No

S11

gi|171677093

Unnamed protein product (aspartate aminotransferase)

5/19%/80

 

MASCOT

43.4/7.6

43.0/8.3

Podospora anserina

No

S12

gi|237868680

Peptidase 1

4/8%/109

KTSGNFIASVASMSLGFESR

KC*FPTEEDRRSGVDVYVVDTGLYTAHSEF

PRO-BLAST

30.9/9.4

38.7/7.9

Pleurotus ostreatus

1.000

S13

gi|255946596

Pc22g00190 (cell wall protein PhiA)

2/9%/81

KGIGYTTGAEPTPK

ASTPKKLYVDR

PRO-BLAST

16.2/4.4

18.9/5.6

Penicillium chrysogenum

0.996

S14

gi|182412796

Hypothetical protein

2/2%/81

FGHWSPNASLATAAK

YGPLSFVKGK

PRO-BLAST

33.1/5.4

74.5/6.8

Opitutus terrae

0.990

S15

gi|261355012

Superoxide dismutase

5/18%/111

GHGLYHDHSLFWENLAC*NLWPK

SYIPPLPYAYDAIEPAISR

PRO-BLAST

22.0/6.3

26.8/10.3

Verticillium albo-atrum

No

S16

gi|189196326

Glutathione reductase

2/4%/90

SFDPIIKDTITKEYER

VAPHYLVLNGSGGLASGR

PRO-BLAST

58.1/7.3

55.8/6.1

Pyrenophora tritici-repentis

No

  1. aThe assignment of the biological or enzymatic function to the hypothetical proteins (based on BlastP analyses) is shown between parentheses and in italic characters.
  2. bNumber of peptides matched/Sequence coverage (%)/Score.
  3. cNN: Non-classically secreted proteins.
  4. dC*: Cys as S carbamidomethyl derivative