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Table 1 Summary of the proteins secreted by D. seriata.

From: Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

Spot Acc. number Protein identity a PM/Seq cov./score b Best peptides (PRO-BLAST) Identified by Experimental MW/pI Theoretical MW/pI Organism Signal P
S1 gi|256724272 Hypothetical protein 2/20%/83   MASCOT 13.2/7.6 13.5/9.6 Nectria haematococca NNc
S2 gi|255935271 Pc13g02210 2/14%/79 KIIYPAYTDK
ARIKGDVVKPDKSYAPKAIP
PRO-BLAST 13.2/7.8 14.5/3.7 Penicillium chrysogenum 0.999
S3 gi|256724272 Hypothetical protein 2/20%/82   MASCOT 13.4/5.6 13.5/9.6 Nectria haematococca NN
S4 gi|27924024 glucosidase 4/11%/97 GFNIGATNADGSC*Kd
EGGHVSISAVSRSEDLYRGDSDASK
PRO-BLAST 30.2/4.7 33.4/9.3 Fusarium sporotrichioides 0.999
S5 gi|256732553 Predicted protein (necrosis and ethylene inducing peptide 2 precursor) 2/7%/97 GELHNDAFALFYAWYFAPG
AVLAHDAVVGFAETVPFVGVWHAHK
PRO-BLAST 35.7/4.7 24.69/7.75 Nectria haematococca 0.999
S6 gi|239931549 Secreted protein 2/9%/105 SGASSWTAAR
SSSFPHWLTLSGSC*NTR
PRO-BLAST 26.3/4.2 23.03/8.35 Streptomyces ghanaensis 0.989
S7 gi|189192036 Hypothetical protein 3/18%/94   MASCOT 19.4/5.1 22.5/7.6 Pyrenophora tritici-repentis 0.999
S8 gi|256726602 Hypothetical protein (necrosis-and ethylene-inducing protein 1) 3/8%/77 FGLFYAWYFPK
ALSPHDSLTKDSYVR
PRO-BLAST 33.8/5.5 26.99/9.2 Nectria haematococca 1.000
S9 gi|156061869 Hypothetical protein (necrosis-and ethylene-inducing protein 1) 6/21%/77   MASCOT 26.0/5.2 26.9/6.6 Sclerotinia sclerotiorum 0.994
S10 gi|189196154 NADP-specific glutamate DH 9/31%/302   MASCOT 48.0/6.9 49.1/5.9 Pyrenophora tritici-repentis No
S11 gi|171677093 Unnamed protein product (aspartate aminotransferase) 5/19%/80   MASCOT 43.4/7.6 43.0/8.3 Podospora anserina No
S12 gi|237868680 Peptidase 1 4/8%/109 KTSGNFIASVASMSLGFESR
KC*FPTEEDRRSGVDVYVVDTGLYTAHSEF
PRO-BLAST 30.9/9.4 38.7/7.9 Pleurotus ostreatus 1.000
S13 gi|255946596 Pc22g00190 (cell wall protein PhiA) 2/9%/81 KGIGYTTGAEPTPK
ASTPKKLYVDR
PRO-BLAST 16.2/4.4 18.9/5.6 Penicillium chrysogenum 0.996
S14 gi|182412796 Hypothetical protein 2/2%/81 FGHWSPNASLATAAK
YGPLSFVKGK
PRO-BLAST 33.1/5.4 74.5/6.8 Opitutus terrae 0.990
S15 gi|261355012 Superoxide dismutase 5/18%/111 GHGLYHDHSLFWENLAC*NLWPK
SYIPPLPYAYDAIEPAISR
PRO-BLAST 22.0/6.3 26.8/10.3 Verticillium albo-atrum No
S16 gi|189196326 Glutathione reductase 2/4%/90 SFDPIIKDTITKEYER
VAPHYLVLNGSGGLASGR
PRO-BLAST 58.1/7.3 55.8/6.1 Pyrenophora tritici-repentis No
  1. aThe assignment of the biological or enzymatic function to the hypothetical proteins (based on BlastP analyses) is shown between parentheses and in italic characters.
  2. bNumber of peptides matched/Sequence coverage (%)/Score.
  3. cNN: Non-classically secreted proteins.
  4. dC*: Cys as S carbamidomethyl derivative