Validation of the upregulation of the tropomyosin (32.9 kDa) protein in wild type N2 after 4 h hypoxia treatment. (A) Western blot analysis showing the upregulation of tropomyosin protein in wild type N2 after 4 h hypoxia treatment compared with the control. The protein α-tubulin (56.3 kDa) was used as an internal control. (B) Image analysis showing that the expression of tropomyosin (LEV-11) is significantly upregulated at 4 h hypoxia compared with the control (P = 0.0069). The expression pattern of LEV-11 was consistent with the quantitative proteomic results.