Figure 5

PS4≡ non-directed activity-based profiling targets several protein classes during HCV replication. (A) Compilation of the 19 true probe targets from Figures 3 and 4 and classification into their corresponding protein family (data from Table 1). (B) Expression analysis of five differentially active protein candidates: heat shock protein 70 kDa (HSP70), protein disulfide isomerase A1 (PDIA1), alpha 2-HS-glycoprotein (AHSG), nuclear distribution gene C homolog (NUDC), and electron transfer flavoprotein alpha polypeptide (ETFA) (Figure 4 and Table 1). The expression analysis was performed by western blotting in the naïve Huh-7 cell line and Huh-7 stably expressing the bi-cistronic pFK-I389neo/NS3-3'/5.1 subgenomic replicon (HCV).