Table 1 Identification of labeled proteins with the PS probe from Figures 3 and 4.
Gel band* | Protein | Function | Accession no. (NCBI) | Mass (kDa) | pI | MASCOT score** | Sequence coverage (%) | Peptide sequence |
|---|---|---|---|---|---|---|---|---|
1 | Protein disulfide isomerase-associated 4 | Isomerase | 4758304 | 72.89 | 4.96 | 57 | 3.4 | -FDVSGYPTIK -IDATSASVLASR |
2 | Heat shock 70 kDa protein 5 | Chaperone | 16507237 | 72.29 | 5.07 | 929 | 36.2 | -VMEHFIK -VLEDSDLK -LTPEEIER -ITITNDQNR -DAGTIAGLNVMR -VEIIANDQGNR -DAGTIAGLNVMR + Ox. of M -FEELNMDLFR -NELESYAYSLK -ELEEIVQPIISK -TWNDPSVQQDIK -SDIDEIVLVGGSTR -TFAPEEISAMVLTK -TFAPEEISAMVLTK + Ox. of M -ITPSYVAFTPEGER -IINEPTAAAIAYGLDK -NQLTSNPENTVFDAK -SQIFSTASDNQPTVTIK -LYGSAGPPPTGEEDTAEKDEL |
3 | Heat shock 70 kDa protein 8 isoform 1 | Chaperone | 5729877 | 70.85 | 5.37 | 767 | 32.5 | -ITITNDK -LSKEDIER -FELTGIPPAPR -DAGTIAGLNVLR -VEIIANDQGNR -YKAEDDLQR -MVNHFIAEFK -FEELNADLFR -NSLESYAFNMK + Ox. of M -SQIHDIVLVGGSTR -TTPSYVAFTDTER -SFYPEEVSSMVLTK -NQVAMNPTNTVFDAK -IINEPTAAAIAYGLDK -NQVAMNPTNTVFDAK + Ox. of M -STAGDTHLGGEDFDNR -TVTNAVVTVPAYFNDSQR |
4 | Heat shock 70 kDa protein 9 | Chaperone | 12653415 | 73.68 | 6.03 | 665 | 22.2 | -VLENAEGAR -RYDDPEVQK -QAASSLQQASLK -DAGQISGLNVLR -VQQTVQDLFGR -AQFEGIVTDLIR -SDIGEVILVGGMTR -TTPSVVAFTADGER -LYSPSQIGAFVLMK -QAVTNPNNTFYATK -LLGQFTLIGIPPAPR -NAVITVPAYFNDSQR |
Gel band* | Protein | Function | Accession no. (NCBI) | Mass (kDa) | pI | MASCOT score** | Sequence coverage (%) | Peptide sequence |
5 | Vimentin | Structural, filament | 340219 | 53.68 | 5.03 | 487 | 24.5 | -QVDQLTNDK -DNLAEDIMR -QDVDNASLAR -FADLSEAANR -VELQELNDR -EYQDLLNVK -ILLAELEQLK -LGDLYEEEMR -NLQEAEEWYK -SLYASSPGGVYATR -ISLPLPNFSSLNLR |
6 | Prolyl 4-hydroxylase, beta subunit precursor (Protein disulfide isomerase) | Isomerase (chaperone) | 20070125 | 57.08 | 4.76 | 619 | 28.5 | - FFPASADR - QLAPIWDK - ENLLDFIK - LKAEGSEIR - VHSFPTLK - ILEFFGLK - THILLFLPK - EADDIVNWLK - LITLEEEMTK - NFEDVAFDEK - MDSTANEVEAVK + Ox. of M - YQLDKDGVVLFK - VDATEESDLAQQYGVR - HNQLPLVIEFTEQTAPK |
7 | Alpha 2-HS-glycoprotein | Ion binding | 156523970 | 39.33 | 5.43 | 136 | 6.3 | -HTLNQIDEVK -CDSSPDSAEDVRK |
8 | ATP synthase beta subunit | Hydrolase | 1374715 | 51.17 | 4.92 | 541 | 32.6 | -IGLFGGAGVGK -IPVGPETLGR -VVDLLAPYAK -TIAMDGTEGLVR -TIAMDGTEGLVR + Ox. of M -IMNVIGEPIDER -IMNVIGEPIDER + Ox. of M -FTQAGSEVSALLGR -TVLIMELINNVAK -VALVYGQMNEPPGAR -VALVYGQMNEPPGAR + Ox. of M -AIAELGIYPAVDPLDSTSR |
9 | Protein disulfide isomerase A5 | Isomerase | 1710248 | 46.17 | 4.95 | 357 | 15.6 | -AATALKDVVK -GESPVDYDGGR -TGEAIVDAALSALR -NLEPEWAAAASEVK -LAAVDATVNQVLASR -GSTAPVGGGAFPTIVER |
10 | Quinolinate phosphoribosyl-transferase | Transferase | 13477197 | 30.81 | 5.82 | 109 | 11.4 | -YDLGGLVMVK -DNHVVAAGGVEK -YGLLVGGAASHR |
11 | Nucleobindin | Ion binding | 189308 | 53.70 | 5.15 | 62 | 2.8 | -LVTLEEFLASTQR |
12 | Phosphoglycerate dehydrogenase | Oxidoreductase | 5771523 | 56.63 | 6.29 | 196 | 10.5 | - TLGILGLGR - GGIVDEGALLR - VTADVINAAEK - ILQDGGLQVVEK - GTIQVITQGTSLK |
Gel band* | Protein | Function | Accession no. (NCBI) | Mass (kDa) | pI | MASCOT score** | Sequence coverage (%) | Peptide sequence |
13 | Aldehyde dehydrogenase 1 | Oxidoreductase | 2183299 | 54.80 | 6.30 | 293 | 11.0 | - ILDLIESGK - VAFTGSTEVGK - QAFQIGSPWR - EEIFGPVQQIMK + oxid(M) - IFVEESIYDEFVR |
14 | Nuclear distribution gene C homolog | Protein binding | 5729953 | 38.22 | 5.27 | 87 | 6.0 | -SETSGPQIK -LVSSDPEINTK |
15 | Cytokine induced protein 29 kDa | Nucleotide, protein binding | 32129199 | 23.66 | 6.10 | 66 | 9.0 | -FGISSVPTK -ITSEIPQTER |
16 | Endoplasmic reticulum protein 29 isoform 1 | Chaperone | 5803013 | 28.97 | 6.77 | 269 | 25.7 | - QGQDNLSSVK - SLNILTAFQK - FDTQYPYGEK - ESYPVFYLFR - GALPLDTVTFYK - DGDFENPVPYTGAVK |
17 | Proteasome subunit alpha type-1 | Hydrolase | 190447 | 30.21 | 6.51 | 143 | 10.6 | -LVSLIGSK -ILHVDNHIGISIAGLTADAR |
18 | Purine nucleoside phosphorylase | Transferase | 230387 | 32.13 | 6.45 | 84 | 9.7 | -FEVGDIMLIR -LGADAVGMSTVPEVIVAR |
19 | Electron transfer flavoprotein, alpha polypeptide | Electron transport | 4503607 | 35.06 | 8.62 | 156 | 16.5 | -SPDTFVR -LEVAPISDIIAIK -GLLPEELTPLILATQK -AAVDAGFVPNDMQVGQTGK |