Figure 1

Tissue treatment groups and stabilization procedure. (A) Brain and lung samples were prepared according to three scenarios typical of the laboratory setting (n = 6/scenario). For liver samples the preparations were expanded to six different conditions potentially encountered in the clinical setting/operating room (n = 6/scenario). (B) Dissected tissue is placed in a hinged Maintainor cassette. The tissue is placed on the flexible Teflon window in the center of the cassette and the cassette is closed. Once placed in the Stabilizer T1 instrument, the air is removed from the cassette by vacuum and subjected to uniform heat and pressure, as described in the Methods.