Figure 2
From: Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization
Preservation of phospho-protein levels in lung and brain samples in laboratory scenarios. SDS-PAGE separated lung (A) and brain (C) total and phospho-protein levels were determined by SyproRuby (total) and ProQ Diamond (phospho) staining, respectively. In both tissues stabilization resulted in increased phospho- to total protein levels as compared to snap frozen control samples. Targeted phosphorylation was examined by immunoblotting using pAktS473 as an example protein. In both the lung (B) and brain (D), pAktS473 to total Akt ratios were higher in the stabilized tissue samples, but did not reach statistical significance. Data are presented as mean ± S.E.M., n = 6/group (* p < 0.05, one way ANOVA with SNK).