Figure 3
From: Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization
Preservation of phospho-protein levels in liver samples in clinical scenarios. (A) SDS-PAGE separated liver total and phospho-protein levels were determined by SyproRuby (total) and ProQ Diamond (phospho) staining, respectively. (B) Stabilization, followed by 2 hours at room temperature prior to cryostorage, resulted in increased phospho- to total protein levels as compared to non-stabilized control samples and samples stored at 4°C for 2 hours. Data are presented as mean ± S.E.M., n = 3/group (* p < 0.05, un-paired t-test). F - snap frozen; DF - Denator, snap freeze; FD - snap freeze, Denator; RT - room temperature; DRT - Denator, room temperature 2 hrs; 4°C - wet ice 2 hrs; D4°C - Denator, wet ice 2 hrs.