Figure 4
From: Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization
Preservation of total phosphorylated tyrosine and phospho-Erk. (A) Stabilization, followed by 2 hours at room temperature or 4°C, resulted in increased phospho- to total protein levels as compared to non-stabilized samples. Data are presented as mean ± S.E.M., n = 6/group (*p < 0.05, paired t-test). (B) Representative blot of phosphorylated tyrosine in immediately stabilized samples followed by 2 hours at room temperature or 4°C compared to control non-stabilized samples. (C) Rapid stabilization increased the amount of phosphorylated Erk detected. Data are presented as means ± S.E.M., n = 5/group. (D) Representative blots of phosphorylated Erk and total Erk in samples stabilized followed by incubation for 2 hours at room temperature or 4°C and control samples. F - snap frozen; DF - Denator, snap freeze; FD - snap freeze, Denator; RT - room temperature; DRT - Denator, room temperature 2 hrs; 4°C - wet ice 2 hrs; D4°C - Denator, wet ice 2 hrs.