Figure 5

Phopsho-Akt levels are significantly higher in stabilized samples. (A) Stabilization of tissue samples followed by incubation at either room temperature or at 4°C yielded greater pAktS473 levels compared to non-stabilized samples. Stabilization resulted in pAktS473 yields comparable to immediate cryopreservation. Data are presented as mean ± S.E.M., n = 5/group (*p < 0.05, **p < 0.005, paired t-test). (B) Representative blots of pAktS473, total Akt, and beta-Actin in samples rapidly stabilized followed by incubation for 2 hours at room temperature or 4°C and control samples stored at room temperature or 4°C for two hours without stabilization. F - snap frozen; DF - Denator, snap freeze; FD - snap freeze, Denator; RT - room temperature; DRT - Denator, room temperature 2 hrs; 4°C - wet ice 2 hrs; D4°C - Denator, wet ice 2 hrs.